fibroblast growth medium 3  (PromoCell)

Journal: bioRxiv

Article Title: Efficient multi-lineage cardiovascular differentiation of human pluripotent stem cells in animal serum-free conditions

doi: 10.64898/2026.01.28.702392

Figure Lengend Snippet: Schematic overview of the workflow developed in this study. Human iPSCs were differentiated into cardiomyocytes (CMs), endothelial cells (ECs), and cardiac fibroblasts (CFs) using serum-free protocols, wherein fetal bovine serum was replaced by Panexin for stromal lineages. For engineered heart tissue (EHT) fabrication, 10% v/v human serum efficiently supported morphological remodelling and functional contractility in CM-only EHTs. Triculture EHTs assembled from CMs (70%), ECs (15%), and CFs (15%) showed enhanced structural organisation and contractile performance under high (10% v/v) and low (1% v/v) human serum conditions in the absence of prolonged TGFβ inhibition by SB431542. Representative contraction traces illustrate functional differences between monoculture and triculture EHTs under optimised conditions.

Article Snippet: Following WNT activation and inhibition phases, cells were replated and matured in fibroblast growth medium (FGM, PromoCell #C-23130) supplemented with Panexin (replacing fetal calf serum), 10 ng/ml FGF2 and 10 μM SB431542, where indicated.

Techniques: Functional Assay, Inhibition